程中一

职称：副研究员

学历/学位：研究生、博士

电话：15370880978

电子邮箱：zyCheng@jiangnan.edu.cn

主要从事酶学与酶工程等方面的研究工作，承担《分子生物学实验》课程的本科教学工作；近年来以第一作者/通讯作者身份发表SCI论文8篇；主持国家自然科学基金青年科学基金、江苏省自然科学基金、中国博士后基金面上项目等多个科研及企业横向项目；指导本科生获全国大学生生命科学竞赛三等奖。

2008-2012   南京林业大学化学工程学院，环境工程专业，工学学士

2012-2019   hjc356黄金城首页线路，发酵工程专业，工学博士

2018-2019   加州大学伯克利分校化学学院     Visiting student

2019-2022   hjc356黄金城首页线路，助理研究员

2022-至今   hjc356黄金城首页线路，副研究员

一、论文（论著）发表情况

1.Cheng Z, Jiang S, Zhou Z. Substrate access tunnel engineering for improving the catalytic activity of a thermophilic nitrile hydratase toward pyridine and pyrazine nitriles. Biochem Biophys Res Commun. 2021 Oct 20;575:8-13.

2.Cheng Z, Zhang W, Xia Y, Ma D, Zhou Z. An anchoring residue adjacent to the substrate access tunnel entrance of a nitrile hydratase directs its catalytic activity towards 3-cyanopyridine. Catal. Sci. Technol., 2021,11, 7525-7529.

3.Cheng Z, Xia Y, Zhou Z. Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications. Front Bioeng Biotechnol. 2020 Apr 24;8:352.

4.Cheng Z, Lan Y, Guo J, Ma D, Jiang S, Lai Q, Zhou Z, Peplowski L. Computational Design of Nitrile Hydratase from Pseudonocardia thermophila JCM3095 for Improved Thermostability. Molecules. 2020 Oct 19;25(20)4.

5.Cheng Z, Peplowski L, Cui W, Xia Y, Liu Z, Zhang J, Kobayashi M, Zhou Z. Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac-mandelonitrile by semi-rational engineering. Biotechnol Bioeng. 2018 Mar;115(3):524-535.

6.Cheng Z, Cui W, Xia Y, Peplowski L, Kobayashi M, Zhou Z. Modulation of Nitrile Hydratase Regioselectivity towards Dinitriles by Tailoring the Substrate Binding Pocket Residues. ChemCatChem. 2018, 10, 449.

7.Cheng Z, Cui W, Liu Z, Wang M, Kobayashi M, Zhou Z. A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards α,ω-dinitriles. Catal. Sci. Technol., 2016,6, 1292-1296.

8.Zhou Z, Ma D, Cheng Z*. Engineering of the thermophilic nitrile hydratase from Pseudonocardia thermophila JCM3095 for large-scale nicotinamide production based on sequence-activity relationships. Int J Biol Macromol. 2021 Nov 30;191:775-782.

9.Ma D‡, Cheng Z‡, Peplowski L‡, Han L, Xia Y, Hou X, Guo J, Yin D, Rao Y*, Zhou Z*. Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis. Chem. Sci., 2022,13, 8417-8428.

10.Hao W‡, Cui W‡, Cheng Z, Han L, Suo F, Liu Z, Zhou L,  Zhou Z*.Development of a base editor for protein evolution via in situ mutation in vivo. Nucleic Acids Research. 2021, 49, 69594–9605.

二、承担教学科研项目情况

1、国家自然科学基金青年基金项目：底物通道入口门控氨基酸调节腈水合酶底物谱的分子机制研究(32101014)，主持

2、中国博士后面上资助：腈水合酶绿色合成手性α-羟基酰胺的对映选择性机制研究（2020M671335)，主持

3、江苏省自然科学基金青年项目：腈水合酶合成手性α-羟基酰胺的对映选择性机制解析（BK20200624)，主持

4、企业横向课题：生产丙烯酰胺的基因工程菌的应用技术转让（200458)，主持

三、获奖情况（含指导学生获奖）

全国大学生生命科学创新创业大赛三等奖指导教师

以上资料更新时间截止：2022年9月